COX-1 vs. COX-2 as a determinant of basal tone in the internal anal sphincter

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COX-1 vs. COX-2 as a determinant of basal tone in the internal anal sphincter.

Prostanoids, produced endogenously via cyclooxygenases (COXs), have been implicated in the sustained contraction of different smooth muscles. The two major types of COXs are COX-1 and COX-2. The COX subtype involved in the basal state of the internal anal sphincter (IAS) smooth muscle tone is not known. To identify the COX subtype, we examined the effect of COX-1- and COX-2-selective inhibitors...

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Internal Anal Sphincter Tone in Humans

39 The extracellular signal which triggers activation of rho-associated kinase (RhoA/ROCK), the major 40 molecular determinant of basal internal anal sphincter (IAS) smooth muscle tone in humans, is not 41 known. Using human IAS tissues, the presence of the biosynthetic machineries for Ang II, TXA2 and 42 PGF2α were identified. These end products of the renin-angiotensin system (Ang II) and ara...

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RhoA/ROCK pathway is the major molecular determinant of basal tone in intact human internal anal sphincter.

The knowledge of molecular control mechanisms underlying the basal tone in the intact human internal anal sphincter (IAS) is critical for the pathophysiology and rational therapy for a number of debilitating rectoanal motility disorders. We determined the role of RhoA/ROCK and PKC pathways by comparing the effects of ROCK- and PKC-selective inhibitors Y 27632 and Gö 6850 (10(-8) to 10(-4) M), r...

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The molecular basis of the genesis of basal tone in internal anal sphincter

Smooth muscle sphincters exhibit basal tone and control passage of contents through organs such as the gastrointestinal tract; loss of this tone leads to disorders such as faecal incontinence. However, the molecular mechanisms underlying this tone remain unknown. Here, we show that deletion of myosin light-chain kinases (MLCK) in the smooth muscle cells from internal anal sphincter (IAS-SMCs) a...

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Role of nitric oxide in beta3-adrenoceptor activation on basal tone of internal anal sphincter.

Effects of activation of beta3-adrenoceptor (beta3-AR) have not been determined in the spontaneously tonic smooth muscle of the internal anal sphincter (IAS). The effects of disodium (R,R)-5-[2-[2-3-chlorophenyl)-2-hydroxyethyl]-amino]propyl]-1,3-benzodioxole-2,2-dicarboxylate (CL 316243), a selective beta3-AR agonist, on the basal smooth muscle tone and direct release of nitric oxide (NO) by c...

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ژورنال

عنوان ژورنال: American Journal of Physiology-Gastrointestinal and Liver Physiology

سال: 2009

ISSN: 0193-1857,1522-1547

DOI: 10.1152/ajpgi.90485.2008